| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092438 |
| Chromosome: | chromosome 6 |
| Location: | 3731672 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278149 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAATGTGAGCAGGCCCTGAGTAGGACG |
| Internal bar code: | TTCGTTCAGGGGGAGACGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 98.8444 |
| LEAP-Seq n confirming: | 5303 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGATATCATGGACCGT |
| Suggested primer 2: | GGCTGTCCTCTGTTTCAAGC |