| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092454 |
| Chromosome: | chromosome 14 |
| Location: | 357633 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGCCATGTGCTTAGCCGCCTTGCGCGC |
| Internal bar code: | GACTAAACACAGGTTCCGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 93 |
| LEAP-Seq percent confirming: | 17.029 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 229 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGCGAAGCTGTGAGTA |
| Suggested primer 2: | AAACGACGACCAGCCATTAG |