Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.092493 |
Chromosome: | chromosome 11 |
Location: | 234197 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467553 | (1 of 1) PTHR23115//PTHR23115:SF162 - TRANSLATION FACTOR // ELONGATION FACTOR G, MITOCHONDRIAL | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCCACACCTCGTCTCTCGCCTGACCCA |
Internal bar code: | GATCTAGGCGTGAAATCTGTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 38 |
LEAP-Seq percent confirming: | 99.2701 |
LEAP-Seq n confirming: | 136 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGGAAGGTGAAGAGGAGAC |
Suggested primer 2: | AGAACAAACTTGTGGGCACC |