Insertion junction: LMJ.RY0402.092532_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.092532
Chromosome:chromosome 3
Location:7491948
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g205500 (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCCAAGCCTCGACGATGTTGTGCTCTT
Internal bar code:TGACCCTGGGGCGAACGGTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:345
LEAP-Seq percent confirming:80.2095
LEAP-Seq n confirming:1378
LEAP-Seq n nonconfirming:340
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGGACACCATCGACAACT
Suggested primer 2:AGGCACTACCACCACCTGTC