Insertion junction: LMJ.RY0402.092542_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.092542
Chromosome:chromosome 4
Location:3936985
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g231124 (1 of 1) PF00397//PF00642 - WW domain (WW) // Zinc finger C-x8-C-x5-C-x3-H type (and similar) (zf-CCCH) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGCACTTTTGCACTTCGCGTCAACTTG
Internal bar code:TGATGTAGAAACCACGCAGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:685
LEAP-Seq percent confirming:98.9318
LEAP-Seq n confirming:5094
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAAACTGTCTTCCGTCTG
Suggested primer 2:CACCTGCAACCACTGCTTTA