| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092553 |
| Chromosome: | scaffold 18 |
| Location: | 32934 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748347 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGTTCAGGACTTGATTATTACTGATTA |
| Internal bar code: | TCTACGGCACATGGCGCGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 99.8095 |
| LEAP-Seq n confirming: | 1048 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAATGAAGTCGTCCTGA |
| Suggested primer 2: | TGGTAGGCATTATGGGGAAG |