Insertion junction: LMJ.RY0402.092554_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.092554
Chromosome:chromosome 7
Location:2104100
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g326150 (1 of 34) IPR000571 - Zinc finger, CCCH-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCGAATCCCTGCCGGCCCCCTCTCGCGC
Internal bar code:CTTGAGTGCGATGTAGAGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:414
LEAP-Seq percent confirming:63.3622
LEAP-Seq n confirming:1828
LEAP-Seq n nonconfirming:1057
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGCCACTCACTCCCTAGC
Suggested primer 2:CGAATTCAGTTTGTCAGCGA