Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.092557 |
Chromosome: | chromosome 17 |
Location: | 1721404 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g708850 | (1 of 226) IPR012337 - Ribonuclease H-like domain | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGCGGCGGCGGCGGCGACAGCAAAGT |
Internal bar code: | GTTGAGCTACGCCAGCTAGGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 208 |
LEAP-Seq percent confirming: | 36.0156 |
LEAP-Seq n confirming: | 555 |
LEAP-Seq n nonconfirming: | 986 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGAGTTGACAACAACCGAGA |
Suggested primer 2: | ATTACCACAGATAACGCGGC |