| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092567 |
| Chromosome: | chromosome 12 |
| Location: | 8107154 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551501 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTCGGCTCGCGGTGCAACTATTCAAAC |
| Internal bar code: | GCGCGGCTAGCCAGACGGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 74.2931 |
| LEAP-Seq n confirming: | 2575 |
| LEAP-Seq n nonconfirming: | 891 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGAGTGCGTGATGTGACT |
| Suggested primer 2: | ATGCAGGGAAACGGATAGTG |