Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.092592 |
Chromosome: | chromosome_17 |
Location: | 1705663 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g708750 | NSG1 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAAGACGTCAGCGCATGAGGGGTGAAGTTG |
Internal bar code: | TGGCGAAGCCCTGCGACTGCGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 152 |
LEAP-Seq percent confirming: | 93.5031 |
LEAP-Seq n confirming: | 1065 |
LEAP-Seq n nonconfirming: | 74 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTAAGCGAGTCCCCTGTCTG |
Suggested primer 2: | ACGGTACGAAGATTGGTTGC |