| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092601 |
| Chromosome: | chromosome 5 |
| Location: | 311963 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233450 | ELG30 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 30 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCGGTAACAACGCGTCTGCTATACCAC |
| Internal bar code: | TGCAGACCCACATGGCATGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 43 |
| LEAP-Seq percent confirming: | 96.7646 |
| LEAP-Seq n confirming: | 3918 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGGCTTCTTCTCTGTGG |
| Suggested primer 2: | TCCCCTATCACAGAACTCCG |