| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092604 |
| Chromosome: | chromosome 10 |
| Location: | 3994845 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448900 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCGGCACCTGCAGCGGAGGTATGAATG |
| Internal bar code: | ACGAGAGATCGAGCGGTGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 99.2819 |
| LEAP-Seq n confirming: | 3318 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGAAATCACCGAAAGGTT |
| Suggested primer 2: | AAGTTCGAGCCAAGCAAAAA |