| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092621 |
| Chromosome: | chromosome 7 |
| Location: | 4933565 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346500 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCCGCTGCTCTGTACTGCTGCCGCGG |
| Internal bar code: | GCGAACCAGACGAGGCATCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 63 |
| LEAP-Seq percent confirming: | 98.9803 |
| LEAP-Seq n confirming: | 3009 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCCAAGTGAGACAAAGC |
| Suggested primer 2: | CTGGTGTCAATGTACGGAGC |