| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092640 |
| Chromosome: | chromosome 10 |
| Location: | 4319138 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450926 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCATGCAACCTCTGCCAGGTGCGCGGG |
| Internal bar code: | GAAAGGAACGCACGTCGGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCTGGCAGATCTGTGTA |
| Suggested primer 2: | ATGTACAACTGCCCTCCGTC |