Insertion junction: LMJ.RY0402.092655_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.092655
Chromosome:chromosome 8
Location:1117734
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g362500 (1 of 1) IPR016024//IPR027165 - Armadillo-type fold // Condensin complex subunit 3 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCTCTGCCATCCCAGCTGCTCCAAGCTG
Internal bar code:GCGCCTTGGAGCAACCGACGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:961
LEAP-Seq percent confirming:99.4644
LEAP-Seq n confirming:9842
LEAP-Seq n nonconfirming:53
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAGCACTTCATTGTCGTA
Suggested primer 2:CACAACGGCTGAAGATTTGA