| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092662 |
| Chromosome: | chromosome_8 |
| Location: | 1019218 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362050 | FAP49 | Flagellar Associated Protein, membrane-associated | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGTCCCCTCAAGGAGGGTCCCGGGGGATT |
| Internal bar code: | CAGATTTAGGGGTAAGAGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 400 |
| LEAP-Seq percent confirming: | 42.4149 |
| LEAP-Seq n confirming: | 137 |
| LEAP-Seq n nonconfirming: | 186 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGTCATGAGAACCTTGA |
| Suggested primer 2: | ACAACGACTGACTCCCCAAC |