| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.092690 |
| Chromosome: | chromosome 10 |
| Location: | 3699934 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446100 | TRX6,TRXY,TRXY1 | Thioredoxin y, chloroplastic; (1 of 1) PTHR10438:SF280 - THIOREDOXIN Y1, CHLOROPLASTIC-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCCAGTGCTAGCCTGAACTGCAAAGCC |
| Internal bar code: | CGGTCGGGTATGTAATGTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.7007 |
| LEAP-Seq n confirming: | 7662 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTTCCTAACCCCACGTA |
| Suggested primer 2: | GCAGTGCAGACCAAGATGAA |