Insertion junction: LMJ.RY0402.092694_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.092694
Chromosome:chromosome 8
Location:1988023
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g367950 FAP323 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like; Cyclic nucleotide-monophosphate binding domain Flagellar Associated Protein 323 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCACACCCTGCCAGTCACCCTTCATGG
Internal bar code:TTTCGCTCGAGGCGGCCAACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:595
LEAP-Seq percent confirming:24.8691
LEAP-Seq n confirming:380
LEAP-Seq n nonconfirming:1148
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TAGAGCTGCAGGTCACATGG
Suggested primer 2:CTGAGAATGTTCTGGGGCAT