| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.092725 |
| Chromosome: | chromosome 8 |
| Location: | 137548 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358538 | (1 of 1) K06443 - lycopene beta-cyclase (lcyB, crtL1, crtY) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCTGGAAGCTGGTGACGCTAGCGAGCT |
| Internal bar code: | ATCTGAGGTCGGAACCTATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1044 |
| LEAP-Seq percent confirming: | 99.5461 |
| LEAP-Seq n confirming: | 16667 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGCCGTGTCAACCCTAC |
| Suggested primer 2: | GAGGGGAAGGAAAAATGAGG |