| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.092738 |
| Chromosome: | chromosome_3 |
| Location: | 2403334 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g159000 | antisense | 3'UTR | ||
| Cre03.g159016 | sense | 3'UTR|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCGTCTGATTCATCATGCACCCTGTTGCA |
| Internal bar code: | GGCAAAATGCGAGTCGGTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1130 |
| LEAP-Seq percent confirming: | 99.7487 |
| LEAP-Seq n confirming: | 397 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTACCAGAGCCACCGTA |
| Suggested primer 2: | TTGTGATGTGTTCCAGGCAT |