| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.092739 |
| Chromosome: | chromosome 6 |
| Location: | 1629008 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260800 | (1 of 1) K05755 - actin related protein 2/3 complex, subunit 4 (ARPC4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCCGATTAATAGTGGCCGTACGACAAG |
| Internal bar code: | CGATGTACGTGTTTTAAGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 99.7417 |
| LEAP-Seq n confirming: | 4248 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCGGCATTCTAAGCAGG |
| Suggested primer 2: | TATTCATAACGCCCCGAGTC |