| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092756 |
| Chromosome: | scaffold 24 |
| Location: | 122720 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g756047 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTCTGTTGCCATTCAAATGCCCAAATT |
| Internal bar code: | GACATACGTAATACCGCGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 94.1222 |
| LEAP-Seq n confirming: | 1217 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCACTCACGAAAATTCA |
| Suggested primer 2: | TGGTCACTTATCACGGTGGA |