| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.092766 |
| Chromosome: | chromosome 17 |
| Location: | 4637718 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g733200 | SRR4 | Scavenger receptor cysteine rich (SRCR) protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTTCGCACTAAGCAATACTGGATTGCG |
| Internal bar code: | GCATCGGTCCTGCTGTGTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 67.404 |
| LEAP-Seq n confirming: | 579 |
| LEAP-Seq n nonconfirming: | 280 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGAACAGTCTCGCAATCA |
| Suggested primer 2: | CAGGCACGGAAACAAGATTT |