| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092781 |
| Chromosome: | chromosome 13 |
| Location: | 3653422 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588736 | (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGCGACTGTAATTGTAAATGAGGCCTG |
| Internal bar code: | AGTTCTAAGCGGGCAAGTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 37.6676 |
| LEAP-Seq n confirming: | 3372 |
| LEAP-Seq n nonconfirming: | 5580 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTTGTTGTTGCTGCTGT |
| Suggested primer 2: | TGGACTATGTGGCTCAGCAG |