| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092793 |
| Chromosome: | chromosome 4 |
| Location: | 2771083 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223750 | (1 of 1) IPR000048//IPR009071//IPR027417 - IQ motif, EF-hand binding site // High mobility group box domain // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCTTTAGCGCTCCATTTCGTGGGCAAG |
| Internal bar code: | GGATCTGTCGGGTCGGTATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 366 |
| LEAP-Seq percent confirming: | 98.493 |
| LEAP-Seq n confirming: | 5490 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTGTGTTCTTCCGTCAC |
| Suggested primer 2: | TAGCTGGCAACATCAACAGC |