| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092826 |
| Chromosome: | chromosome 4 |
| Location: | 3733547 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229350 | (1 of 1) 2.1.1.290 - tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase / tRNA-yW synthesizing enzyme-4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAATCAGGACGGCGCAAAAGCGAGCGAAC |
| Internal bar code: | GGGAAGAGTCCGGTTAGGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 99.0899 |
| LEAP-Seq n confirming: | 4464 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCAACTTGTGTTCCAGGC |
| Suggested primer 2: | GGAGCTCGTCCTGCTATACG |