Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.092827 |
Chromosome: | chromosome 1 |
Location: | 6997811 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g050600 | (1 of 4) 2.1.1.62 - mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGTGCTGGAGTGCTGGGGCGCTGGGGC |
Internal bar code: | ACGTGGTTGGCCCTTGTGTGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1016 |
LEAP-Seq percent confirming: | 99.6493 |
LEAP-Seq n confirming: | 23871 |
LEAP-Seq n nonconfirming: | 84 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCATTCTCTACCCAGCTCCA |
Suggested primer 2: | AGGGAGTGTTACGGCATTTG |