| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092827 |
| Chromosome: | chromosome 1 |
| Location: | 6998256 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050600 | (1 of 4) 2.1.1.62 - mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGCGGCGCCATGCTAAGGAGATAACAC |
| Internal bar code: | TTGGGCATGTGTAGTCATATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 99.3249 |
| LEAP-Seq n confirming: | 8386 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGAGGGCAATACTGGGT |
| Suggested primer 2: | TCTTTCCGCTCCACAGAAGT |