| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092859 |
| Chromosome: | chromosome 15 |
| Location: | 902851 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639400 | (1 of 2) 2.6.99.4 - Transferred entry: 2.3.1.234 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTAATGAAGAAGTCGCAGCGAAGGCAGG |
| Internal bar code: | GACAAAGGACCGAGGCTCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 23 |
| LEAP-Seq percent confirming: | 99.1342 |
| LEAP-Seq n confirming: | 687 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAGCCATCATTCAGACC |
| Suggested primer 2: | GTGAGGTGGTTGAGACGGTT |