Insertion junction: LMJ.RY0402.092860_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.092860
Chromosome:chromosome 2
Location:6847896
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g119450 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACAAGAACCCGGACATGCTTGTGTCCAC
Internal bar code:GCGGGTGTCAGGGGGCTCGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:579
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGTAACAGCGTGTCCACCTT
Suggested primer 2:AGCTCTCTCACGCTCCTCTG