| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092904 |
| Chromosome: | chromosome 16 |
| Location: | 7002366 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677765 | (1 of 4) PF00207 - Alpha-2-macroglobulin family (A2M) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTTGGCAAACCCGCGTGACTTGCTGTT |
| Internal bar code: | TTCTGCAGGGGAGTCGACCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 96.831 |
| LEAP-Seq n confirming: | 550 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTGGAGGTCACACCGTT |
| Suggested primer 2: | TGCATCTGAACTTTTCGTGC |