| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092919 |
| Chromosome: | chromosome 1 |
| Location: | 5135321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035750 | MSC3 | Mechanosensitive ion channel; (1 of 3) PTHR30566 - YNAI-RELATED MECHANOSENSITIVE ION CHANNEL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACCTTGTAGAGTGTCATCAGTCCCTGT |
| Internal bar code: | GCTTTGACCGGGGGTGTACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 98.8353 |
| LEAP-Seq n confirming: | 4837 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGCTGCTGGCTTACCGAA |
| Suggested primer 2: | TTGTGCTGCGTCAACCTTAC |