| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092923 |
| Chromosome: | chromosome 12 |
| Location: | 4591825 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522600 | CYC1,CYC2,CYC | (1 of 1) K08738 - cytochrome c (CYC); mitochondrial cytochrome c | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGGATTCCGGCGCGGTCTGACCGTTGT |
| Internal bar code: | TAGCTAGGGAGGTCTTACTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 99.1884 |
| LEAP-Seq n confirming: | 1711 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTGGGCTGTTACCAAA |
| Suggested primer 2: | TTTCAAAAGCTGCGTGAATG |