Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.092926 |
Chromosome: | chromosome 2 |
Location: | 9164774 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g143507 | 3'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGGGGGGACAGGAGGGGCAAGGAGAGGG |
Internal bar code: | CCCCAGCTCGAGCGACAGCCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 577 |
LEAP-Seq percent confirming: | 97.629 |
LEAP-Seq n confirming: | 1400 |
LEAP-Seq n nonconfirming: | 34 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGTGTGTGGGTGTGTGTCA |
Suggested primer 2: | GCTAGACCACTAAGCACGCC |