| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.092934 |
| Chromosome: | chromosome 10 |
| Location: | 1356733 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427850 | (1 of 1) IPR000014//IPR000104//IPR013767 - PAS domain // Antifreeze protein, type I // PAS fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGGGGGCTGTGCGCTGGCCGTTGGCAC |
| Internal bar code: | GAGATCTCTCGTTGATCAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 55.8214 |
| LEAP-Seq n confirming: | 1213 |
| LEAP-Seq n nonconfirming: | 960 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACATGATTGACGATGAGG |
| Suggested primer 2: | CCAGGACGTTCATGTTGTTG |