| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092939 |
| Chromosome: | chromosome 2 |
| Location: | 7117994 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGGTGGCAATGGTGGGGATGCCGATG |
| Internal bar code: | ACACGGACTCGGTTCTCGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 65.1877 |
| LEAP-Seq n confirming: | 573 |
| LEAP-Seq n nonconfirming: | 306 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCGGCACTACCTTGACC |
| Suggested primer 2: | GATGGACGTTGAGGTGGAGT |