Insertion junction: LMJ.RY0402.092945_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.092945
Chromosome:chromosome 16
Location:7484101
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g687182 POB10 Proteome of basal body 10; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCTATCCATCGCAGCCGTACGACCTGAG
Internal bar code:GCACATTGAATGTCCCGGCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:769
LEAP-Seq percent confirming:99.4533
LEAP-Seq n confirming:2183
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTACTTGTGAGGGCTTGC
Suggested primer 2:CTCACAGCTTATGATCCGCA