| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.092951 |
| Chromosome: | chromosome 16 |
| Location: | 7150362 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680902 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGGTCAACCGTTGCTTGTTATCTATAG |
| Internal bar code: | GCGGCCTCGTTTGCCTGCTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 410 |
| LEAP-Seq percent confirming: | 99.5099 |
| LEAP-Seq n confirming: | 5076 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCGACTTTGACTGGACA |
| Suggested primer 2: | CTGGCAGCTCAACACACAGT |