| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.092957 |
| Chromosome: | chromosome 14 |
| Location: | 658480 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612250 | FAP388 | Flagellar Associated Protein 388 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGATGATTATGAAAGCAAGGTTGGTCGTT |
| Internal bar code: | TTTGTTCAATGCCTTAAACATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 234 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGGCAGTGAGCAGGATG |
| Suggested primer 2: | CGTGCAACTGTGTGTGTGAG |