| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092967 |
| Chromosome: | chromosome 1 |
| Location: | 703108 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003700 | (1 of 2) PF10263 - SprT-like family (SprT-like) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGGGGCAAGCACCCAGCGGGTCGGCTG |
| Internal bar code: | AGGCGCCACGGGGTGGACGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 355 |
| LEAP-Seq percent confirming: | 99.6622 |
| LEAP-Seq n confirming: | 295 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCAACATCACGAACACG |
| Suggested primer 2: | TACGGCTCCGTACAAATCGT |