Insertion junction: LMJ.RY0402.092991_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.092991
Chromosome:chromosome 3
Location:2038378
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g156250 FAP186,POC13 Proteome of centriole protein 13; (1 of 5) PF04366 - Las17-binding protein actin regulator (Ysc84) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCAAGCCTCCTCGTAGCTTACCGCGCCG
Internal bar code:TCTTTTCTCTTCAATGTGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:834
LEAP-Seq percent confirming:99.8105
LEAP-Seq n confirming:2107
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGAACCTCAATGGGTCG
Suggested primer 2:GGCGGTGACTTCTTTCTCTG