Insertion junction: LMJ.RY0402.093000_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.093000
Chromosome:chromosome 17
Location:6202464
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCCGAATCCCCCCCCCGCTCAGGCATTT
Internal bar code:GTCGTGCGCCTGCTACATGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:718
LEAP-Seq percent confirming:99.3449
LEAP-Seq n confirming:11374
LEAP-Seq n nonconfirming:75
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTCCATAGTCTCCAGCG
Suggested primer 2:CCCAGGATTTACAAGGGGAT