Insertion junction: LMJ.RY0402.093000_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.093000
Chromosome:chromosome 17
Location:6202559
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGAGGGAATTGCGTCTTTCACTTACAAGC
Internal bar code:CTATAGGGGCCTTAGCTTCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:589
LEAP-Seq percent confirming:74.0568
LEAP-Seq n confirming:10325
LEAP-Seq n nonconfirming:3617
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTCCATAGTCTCCAGCG
Suggested primer 2:CCCAGGATTTACAAGGGGAT