| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093019 |
| Chromosome: | chromosome 1 |
| Location: | 2639967 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015400 | (1 of 2) PF05542 - Protein of unknown function (DUF760) (DUF760) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCTCTACTTTTACTTCTTCACTACCG |
| Internal bar code: | ATGAAAACTATTGGACCGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 42 |
| LEAP-Seq percent confirming: | 99.1582 |
| LEAP-Seq n confirming: | 589 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCCGCCTACATAGACAT |
| Suggested primer 2: | ACGTAAGCACACAGCCACAG |