| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093040 |
| Chromosome: | chromosome 7 |
| Location: | 1821320 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325728 | (1 of 5) PF12681 - Glyoxalase-like domain (Glyoxalase_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGGGTGCCCAGCACCCAAGTCAAGATA |
| Internal bar code: | CTAGAGCCCTGGGGCACCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 96.6178 |
| LEAP-Seq n confirming: | 3428 |
| LEAP-Seq n nonconfirming: | 120 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTATGGTACCGCGTTTT |
| Suggested primer 2: | GTTTGGTAAAGGCAGTGGGA |