| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093058 |
| Chromosome: | chromosome_13 |
| Location: | 1505520 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g572700 | FLA15,FAP66,IFT144 | Intraflagellar transport protein 144 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGAAGGGTGGGGGCCAGGAGGCAGCCGAGT |
| Internal bar code: | GCTAGTACTGTGGGTGAAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 172 |
| LEAP-Seq percent confirming: | 20.5694 |
| LEAP-Seq n confirming: | 1156 |
| LEAP-Seq n nonconfirming: | 4464 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCCTCTGTCATCTGCT |
| Suggested primer 2: | AAGAGCGGAGCACTCACATT |