| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093103 |
| Chromosome: | chromosome_9 |
| Location: | 7820673 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g416550 | FAP193,MBO2 | Coiled-coil flagellar protein, Move Backward Only 2 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGTTCTGCAGGATGTCCAGCTCGTTCTGA |
| Internal bar code: | TGTCTAGAACCTAACCAGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 93.7757 |
| LEAP-Seq n confirming: | 2742 |
| LEAP-Seq n nonconfirming: | 182 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTCCAGCTCGTGCTATG |
| Suggested primer 2: | CTAGAACCTGGCACTGGAGC |