| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093119 |
| Chromosome: | chromosome 3 |
| Location: | 4554455 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176800 | FPG1,FPGS1 | (1 of 1) 6.3.2.12 - Dihydrofolate synthase / H(2)-folate synthetase; Putative folylpolyglutamate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGCGGCAGCACCCGTCGGAGGCGGCAC |
| Internal bar code: | ATGCCTGCGCAGGCTCGACTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 74.7126 |
| LEAP-Seq n confirming: | 260 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGTACTTGGGGTGCTGC |
| Suggested primer 2: | CCTTGTGCTCCGCTAGTTTC |