| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093138 |
| Chromosome: | chromosome 7 |
| Location: | 1692274 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325600 | GRX5 | Glutaredoxin, CGFS type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTAAAAGGGCCGGTGCGCAACACGGAC |
| Internal bar code: | CGCTTTGGGGTGGTTAGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 532 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTTATCAACCGACGATG |
| Suggested primer 2: | CTTGGCAGAAACCATCCCTA |