| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093148 |
| Chromosome: | chromosome 3 |
| Location: | 238717 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144404 | (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCTCAACCTGCATGCATGGGAGCACAC |
| Internal bar code: | TAGAAATTCACTAAGTGTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 97.8423 |
| LEAP-Seq n confirming: | 5260 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAGTTGTCACCTTGAGT |
| Suggested primer 2: | GAGGCTCGTAAAACACCTGC |